General Cell Lines and Cell Cultures
STEMCELL Technologies G-CSF Mobilized Human Peripheral Blood Mononuclear Cells, Frozen, Size: 5 x 10^6 cells
Start your assays with ready-to-use, ethically sourced, mobilized primary mononuclear cells. We help you get the cells you need with personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications. Donors are mobilized with G-CSF, plerixafor, or G-CSF and plerixafor prior to collection. Isolated from leukapheresis samples using density gradient separation or red blood cell lysis and cryopreserved in CryoStorCS10 medium, cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. ACDA is added as an anticoagulant. Contact techsupport@stemcell.com for donor specifications (BMI category, smoking status, ethnicity, etc.). Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. Certain products are only available in select territories.
STEMCELL Technologies Human Bone Marrow CD34+ Cells, Frozen, Size: 3 x 10^5 cells
Start experiments confidently with ready-to-use, ethically sourced, CD34+ stem and progenitor cells from adult human bone marrow. With personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications, we help you get the cells you need. Isolated immunomagnetically using positive selection and cryopreserved in serum-free medium, cells are collected using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation to meet institutional requirements and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Heparin is added during collection as an anticoagulant. Contact techsupport@stemcell.com for donor specifications (BMI category, smoking status, ethnicity, etc.). Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. Certain products are only available in select territories.
STEMCELL Technologies Human Peripheral Blood B Cells, Frozen, Size: 2 x 10^7 cells
Human primary B cells derived from peripheral blood (PB) are isolated from mononuclear cells (MNCs) using the negative immunomagnetic selection technique. Cells are untouched and ready for downstream applications. PB was collected using acid-citrate-dextrose solution A (ACDA) as the anticoagulant.Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.Certain products are only available in select territories. Please contact your local Sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.Browse our Frequently Asked Questions (FAQs) (https://www.stemcell.com/primary-cells-faq) on Primary Cells.
Eurofins Discoverx PathHunter CHO-K1 GPR75 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0363C2A)
The PathHunter GPR75 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR75 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR75 and EA-tagged β-Arrestin-2. Activation of GPR75 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx cAMP Hunter CHO-K1 LHCGR Gs Cell Line, 2 vials. (95-0106C2)
The cAMP Hunter LHCGR Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. luteinizing hormone) based activation of LHCGR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type LHCGR, a naturally Gs coupled GPCR. Upon activation of LHCGR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS TORC3 Nuclear Translocation Cell Line, 2 vials. (93-1120C3)
The PathHunter U2OS TORC3 Nuclear Translocation Cell Line is a stable, engineered cell line for use in studying drug candidates affecting the CRTC/CREB pathway. This cell line enables assessment of ligand (e.g. forskolin) based pathway activation via detection of resulting TORC3 (CRTC3) nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged TORC3 (CRTC3) and nuclear localized EA. Upon pathway activation, induced nuclear translocation of TORC3 leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/pathways.
Eurofins Discoverx PathHunter CHO-K1 NMBR β-Arrestin Cell Line, 2 vials. (93-0925C2)
The PathHunter NMBR cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. neuromedin B) based activation of NMBR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NMBR and EA-tagged β-Arrestin-2. Activation of NMBR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx Ready-to-Assay 5-HT2C Serotonin Receptor Frozen Cells, 2 Vials of Cells. (HTS132RTA)
The Ready-to-Assay 5-HT2C Serotonin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. serotonin/5-HT) based activation of HTR2C GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HTR2C alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of HTR2C, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay CXCR1 Chemokine Receptor Frozen Cells, 2 Vials of Cells. (HTS001RTA)
The Ready-to-Assay CXCR1 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CXCL8/IL8) based activation of CXCR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CXCR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CXCR1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen 5-HT1B Serotonin Receptor Membrane Preparation, 1 vial. (HTS108M)
The ChemiScreen 5-HT1B Serotonin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of HTR1B surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay CCR1 Chemokine Receptor Frozen Cells, 2 Vials of Cells. (HTS005RTA)
The Ready-to-Assay CCR1 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CCL2, MCP-1) based activation of CCR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CCR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCR1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay SST5 Somatostatin Receptor Frozen Cells, 2 Vials of Cells. (HTS139RTA)
The Ready-to-Assay SST5 Somatostatin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. somatostatin) based activation of SSTR5 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses SSTR5 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of SSTR5, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Creative Biolabs Human OVCAR-8 Tumor Cell Line, Tumor Cell Line, OVCAR-8, Ovarian Adenocarcinoma, Epithelial, Adherent morphology, Storage: Liquid nitrogen (-150°C to -190°C), Applications: Cancer research, Drug screening
Human OVCAR-8 Tumor Cell Line, Tumor Cell Line, OVCAR-8, Ovarian Adenocarcinoma, Epithelial, Adherent morphology, Storage: Liquid nitrogen (-150°C to -190°C), Applications: Cancer research, Drug screening
As One International Inc Green Fluorescent primary HUVEC - tube formation assay cells, 3 x 10^6 cells
Green Fluorescent primary HUVEC - tube formation assay cells, 3 x 10^6 cells
Cell Biologics ESOPHAGEAL EPITHELIAL CELL-HU
HUMAN PRIMARY ESOPHAGEAL EPITHELIAL CELLS - Frozen Vial